Anyone tried the DIY TRIIM protocol? HGH + retatrutide + DHEA for actual age reversal

Interesting thread. I've been on GH for about 18 months for recovery and sleep and hadn't thought about the longevity angle at all. DHEA is something my doctor has mentioned before for my age. Would be curious whether adding it on top of GH makes a noticeable difference or whether it's too subtle to detect without proper tests.

Been down the rabbit hole on the TRIIM study lately and figured I'd see who else is actually running this or something similar.

For those who haven't read it - 9 guys ran HGH + metformin + DHEA for a year and showed roughly 2.5 year biological age reversal on the GrimAge epigenetic clock. Thymus regeneration was the standout finding, immune markers improved, and insulin sensitivity held up throughout. Small sample but the mechanism stacks up.

Most people running the DIY version now are swapping metformin for retatrutide anyway. Better insulin sensitisation and you get the fat loss on top, which makes more sense for our context than straight metformin does.

Anyone else running HGH alongside a GLP-1 in a longevity context rather than just for prep? Curious what DHEA dose people are landing on too - I've seen everything from 50mg to 100mg per day thrown around.

The epigenetic clock data is interesting but 9 participants is barely a case series, let alone a study you can draw conclusions from. Horvath clocks have shown variance even in much larger trials and a lot of researchers in the longevity space are cautious about treating the reversal numbers as literal biological age. I think the honest read is we don't know yet.

That said, I'm running GH at 2 IU fasted AM regardless, so adding 50mg DHEA in the morning is not exactly a leap. Sourced mine from a standard supplement brand, nothing fancy. The retatrutide piece is the one I'd want more community data on before committing to it long term.

interesting thread. the injection frequency fatigue is a real thing when you start stacking HGH with multiple peptides on top - I have seen guys drop protocols midway just because the daily pin schedule became unsustainable. worth factoring in before committing to the full stack.

the 9-participant study is what trips me up every time someone brings this up. statistically it means basically nothing, and whether GrimAge accurately predicts actual lifespan extension is still debated even by the researchers who ran it. the thymus regeneration finding is harder to dismiss though - that is measurable immune output, harder to wave away than epigenetic clocks. my issue with the full DIY stack is the injection burden. if you are already running test plus GH nightly, adding reta twice weekly on top puts you at 10+ pins a week minimum. at some point it stops being a longevity protocol and starts being a part-time job.

Interesting stuff. We've had a few customers running similar DIY versions - the main thing I hear is injection fatigue when they stack too many peptides together. The skepticism about the sample size is fair but the mechanism is sound. Curious to see where this goes over the next few years.

The 9-participant study thing is always the first thing skeptics bring up and honestly they're not wrong to flag it. GrimAge as a lifespan predictor hasn't been validated in anything close to a serious sample size. That's just the reality.

But the thymus regeneration data is harder to dismiss. T-cell production improving is measurable and has actual immune function consequences, not just a number on a clock shifting. That part of the study stacks up better when you dig into it.

For me the calculation is simple - HGH, DHEA and reta are compounds most of us are already comfortable running. If the downside is that the longevity angle turns out to be overblown, I've still been running GH at 3IU fasted AM which I'd be doing anyway. The DHEA and reta are just on top. Not exactly a massive leap of faith.

Been following this one. The thymus regeneration finding interests me more than the GrimAge headline. Nine participants is barely a pilot study and epigenetic clocks have their own methodological critics. Still, plenty of guys here were running HGH plus DHEA long before that paper came out, so the stack itself is not exactly new territory. Swapping metformin for reta makes sense for the insulin sensitiser piece, and the injection load is the honest concern - once you are pinning GH, reta twice a week, and anything else on top it starts feeling like a part-time job.

The skepticism around the TRIIM study is fair given the sample size. Nine participants is not enough to draw firm conclusions from. That said, the components individually are well-studied and the stack carries a reasonable safety profile at the doses being discussed. Worth monitoring the longer-term data as it comes in.

The skepticism around epigenetic clocks is fair but I think people are missing the point when they use it to dismiss the whole protocol. Even if the clock validity is contested, the actual markers from the TRIIM study were solid - thymus regeneration, improved T-cell counts, maintained insulin sensitivity. That stuff is measurable and not dependent on whether the biological age calculation is accurate. Running 3 IU HGH pre-bed plus 50mg DHEA and using reta instead of metformin - the injection burden is there but compared to a full blast it's nothing. Happy to track markers over 6 months if others are doing the same.

The injection schedule is honestly the part that gets me with the full TRIIM stack. Running GH, reta twice a week, and DHEA on top - you're halfway through your morning just managing vials and pins. I dropped Mots-C a while back for exactly this reason, benefit wasn't clear enough to justify another jab. Anyone doing the full protocol, what timing are you using for the GH? Switched mine to pre-bed and fasted glucose on my bloods came back clean - was getting flagged before on fasted AM shots.

Good thread. The skepticism around epigenetic clocks is fair given the sample sizes but I'd rather be running this stack than not while we wait for bigger trials.

Running the DIY version myself right now - 3IU HGH pre-bed, reta at 1mg/week split Mon/Thu, 50mg DHEA daily. Only 8 weeks in so nothing dramatic to report on the epigenetic side but sleep quality has noticeably improved and body comp is slowly shifting without touching my diet.

The injection burden is real though. GH every night plus reta twice a week on top of whatever else you're already pinning - after a while you're doing a mental tally of how many jabs you're running. DHEA is at least just a capsule, grab it from any health store, no issues there.

The study skepticism is fair - 9 people is statistically nothing. But the mechanism makes sense and if you're already running GH the marginal cost of adding reta and DHEA is pretty low. Worst case you learned something.

Similar stack here last year - HGH 3iu before bed plus DHEA 75mg, no reta at that point. Adding reta this year and the injection schedule is getting real. GH daily plus twice weekly reta adds up more than you think going in, and if you're running any other peptides on top you're pinning constantly. At least DHEA is caps, one less thing to draw up. Mildly skeptical on the epigenetic clock stuff too - 9 participants is basically a case series and GrimAge as an actual lifespan predictor has real limitations the paper acknowledges.

Good discussion in here. The injection burden point is real and gets underestimated when people first map out a longevity stack on paper. HGH plus reta plus DHEA plus whatever else is already 3-4 compounds before you add anything for training. Worth being honest with yourself about what you will actually sustain for 12 months versus what looks good in a spreadsheet.

The 9-participant thing bothers me every time I look at this study. Still running a version anyway - 3IU GH pre-bed, 1mg reta split Mon/Thu, 50mg DHEA daily. Risk profile is reasonable and the compounds make sense independently of the aging claims. The epigenetic clock data is interesting but those clocks aren't validated as actual lifespan predictors. Biological age on paper and how long you actually live aren't the same number.

The injection fatigue is real on these longevity stacks. GH twice daily plus a GHRP plus retatrutide plus DHEA oral - you're adding 4-5 more decisions and prep steps to every day. I cut it back to 3 IU GH pre-bed plus 50mg DHEA and dropped the rest for now. Running it properly for the full year like the TRIIM study takes more discipline than most people expect.

Reading along on this one. The thymus regeneration finding is what I keep coming back to, the rest is interesting but that one has practical implications well beyond bodybuilding. Good to see the community running it sensibly.

Started my own version of this 6 weeks ago, 3IU GH pre-bed, 1mg reta weekly, 75mg DHEA. The reta replacing metformin makes practical sense, you get the insulin sensitivity plus 4kg off in the first month I wasn't planning for. Skeptical about the GrimAge numbers being meaningful for actual lifespan but the bloodwork shifts are real, fasting glucose 79 from 92, IGF-1 climbing to 290, lipids cleaned up. Worth running for 12 months and seeing what the second panel says.

Been running a version of this since February. 3IU pre-bed instead of fasted AM, 1mg reta split twice weekly, 75mg DHEA first thing. The injection load honestly is not the problem people make it out to be. Sleep is noticeably better and recovery between sessions has shifted, hard to put a number on it but something is different. I am not pulling epigenetic clocks to validate it, if the quality of life markers are moving the right direction that is enough to keep going.